Coding

Part:BBa_K4488026:Design

Designed by: Jessica Zhang   Group: iGEM22_Sydney_Australia   (2022-10-04)


Fusion of free-use GFP-alanine with CBDcipA (cellulose-binding domain) at the C-terminal end with a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1361
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Needed to add a linker, remove banned restriction sites.


Source

fuGFP

References